The Gamma-GluTamyl TranspepTidase ConTenTs in serum and urine in newborn as an indiCaTor of disTurbanCe Kidney funCTion due To asphyxia

The aim of the investigation was to evaluate the diagnostic value of the study serum and urinary contents of γ-glutamyl transpeptidase (GGTP) in newborns with impaired renal function due to asphyxia. Materials and Methods. Investigation included 200 full-term newborns with disturbance kidney function: 100 infants who had severe asphyxia, and 100 — with moderate asphyxia. Comparison group consisted of 20 healthy children without asphyxia. The peripheral venous blood and urine of newborns were picked up by 1–2, 7–8 and 25–30 days of life. GGTP activity was determined by a standardized method of “endpoint” after rate of formation of 5-amino-2-nitrobenzoate at 405 nm and 37°C on the semi-automatic photometer using reagents of Olvex Company (Russia). Results. Disturbance kidney function in neonates with asphyxia causes a significant increase the level of GGTP in serum and urine in the first 24–48 hours after birth. Enzyme concentration in biological fluids during this period in the groups of surveyed children was the highest. In the dynamics of the early neonatal period marked reduction of GGTP in serum and urine in children with kidney dysfunction due to asphyxia. By the end of the neonatal period the enzyme content in serum and urine in neonates with asphyxia and disturbance kidney function remained high. In infants with renal impairment due to moderate asphyxia during the first weeks of life ratio of serum/urine GGTP content decreased less than 2.5 relative to the comparison group, followed its raised to 25–30 days of life. At the same time, infants with severe renal impairment due to asphyxia were characterized by high rates ratio (greater than 4.0) during the whole neonatal period. In the examined groups of children during the early neonatal period indicated a statistically significant positive moderate correlation between the serum and urinary GGTP content. Conclusion. Serum GGTP activity is characterized severity of cytolysis syndrome of proximal renal tubular epithelium in neonates with disturbance kidney function due to asphyxia. Noninvasive determination of GGTP levels in the urine in the early neonatal period, preferably for the screening of renal problems in neonates with asphyxia.